Scientific Staff

Dr Pradeep Kumar Sharma
Environmental Carcinogenesis
CSIR-Indian Institute of Toxicology Research
Vishvigyan Bhavan, 31, Mahatma Gandhi Marg,
Lucknow - 226 001. Uttar Pradesh, India
Email: pradeep.sharma[at]iitr[dot]res[dot]in | Alternate Email:
Tel: 522-2613786 | Extension: 308
Fax: 522-2628227
Area of Specialization
Environmental carcinogenesis, cancer biology and Endocrine disrupting chemicals-mediated toxicity.
Current R&D/S&T Activities

During PhD, I worked on selective radio sensitization of cancer cells using metabolic modifiers. Using various cellular and molecular techniques we were able to show that simultaneous inhibition of glycolysis (by using 2-deoxy-D-glucose) and pentose phosphate pathway (by using 6-aminonicotinamide) sensitize cancer cells selectively towards radiation induced damage.

During postdoctoral research work, I worked on elucidating the molecular mechanism underlying homocysteine mediated neuronal cell death. Both in established continuous cell lines (neuroblastoma origin) and rat primary cortical neurons, Homocysteine accumulation resulted in ER stress as an early event which further culminated in to neuronal cell death at later stage by cross talking to mitochondria. Moreover, we identified that homocysteine treatment resulted in the suppression of autophagy which is a survival mechanism that maintains cellular homeostasis and quite predominant in terminally differentiated cells such as neurons. We also validated our in vitro findings at organism level using zebra fish embryos as a model system. By employing molecular and genetic approaches we observed striking similarities in both in vitro and in vivo model system and proposed that suppression of autophagy is a crucial event of molecular mechanism underlying homocystein mediated neuronal cell death. 

Partial List of Research Publications
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