Current R&D/S&T Activities
Microglial cells are modified macrophages that protect central nervous system (CNS) from infections and other assaults to it. Its activities begin in the early weeks after birth and continue thereafter. Effect on its development and functions in early life can therefore be detrimental to neuronal activity of affected individual. Microglia acts as double edged sword. In resting state microglia helps in neuronal survival by secreting trophic factors (BDNF, IGF) and anti-inflammtory molecules (IL-10 and TGF-β). On the other hand, over activation of microglia exerts detrimental effect by releasing high amount of pro-inflammatory factors like superoxide, nitric oxide, IL-1β, IL-6 as well as TNF-α.
Environmental chemicals, such as lead, trimethyltin and diesel exhaust potentially hyperactivate microglia and induce neuronal anomalies. Our lab is focused to find out the molecular mechanisms of xenobiotic induced altered immune function of microglia.
# Peer reviewed articles:
MP, Verma NK, Kar AK, Singh A, Ghosh
D, Patnaik S. (2017) Inhibition of Thioredoxin Reductase
by Targeted Selenopolymeric Nanocarriers Synergizes the Therapeutic Efficacy of
Doxorubicin in MCF7 Human Breast Cancer Cells. ACS Appl Mat Inter. 9; 36493-36512.
G, Singh V, Dewangan J, Daniel P V, Kamthan M, Ghosh D*,
Mondal P*, Ghosh S*. (2017) Renal clearable new NIR probe: precise
quantification of albumin in biofluids and fatty liver disease state
identification through tissue specific high contrast imaging in vivo. Anal
Chem. 89; 10343-10352.
3. Gera R, Singh V, Mitra S, Sharma AK, Singh A,
Dasgupta A, Singh D, Kumar M, Jagdale P, Patnaik P, Ghosh D*. (2017) Arsenic exposure impels CD4 commitment in
thymus and suppresses T cell cytokine secretion by increasing regulatory T
cells. Sci. Rep. 7; 7140.
V, Gera R, Purohit MP, Patnaik S, Ghosh
Fluorometric estimation of Glutathione in Cultured Microglial Cell
Lysate. Bio-protocol. 7, 1-9, DOI:10.21769/BioProtoc.2304.
AK; Singh V; Gera R; Purohit MP; Ghosh D*. (2016) Zinc Oxide
Nanoparticle Induces Microglial Death by NADPH-Oxidase-Independent Reactive
Oxygen Species as well as Energy Depletion. Mol Neurobiol. DOI 10.1007/s12035-016-0133-7.
V, Gera R, Kushwaha R, Sharma AK, Patnaik S, Ghosh D*.(2016) Hijacking microglial
glutathione by inorganic arsenic impels bystander death of immature neurons
through extracellular cystine/glutamate imbalance. Sci. Rep. 6; 2016;
V, Mitra S, Sharma AK, Gera R, Ghosh D*. (2014) Isolation and
characterization ofmicroglia from adult mouse brain: Selected applications for ex-vivo
evaluation of immunotoxicological alterations following in-vivo xenobiotic
exposure. Chem Res Toxicol. 27, 895−903.
# Book Chapter:
1. Gera R, Singh V, Sharma AK, Ghosh D*. (2015) Environmental Chemicals: Insights into Mammalian Immune Responses to Arsenic, Cadmium, and Lead. Adv Mol Tox.9, 109-159. [Book series published by ELSEVIER].
[* denotes corresponding author]